Section NR 252.035 - Potassium ferricyanide titration methodThe following method is based on method SLM 4/2 described in Official Method of Analysis, Society of Leather Trades' Chemists, Fourth Revised Edition, Redbourn, Herts., England, 1965 and is to be used for the determination of sulfide in alkaline wastewater.
(1) OUTLINE OF METHOD. (a) The buffered sulfide solution is titrated with standard potassium ferricyanide solution in the presence of a ferrous dimethylglyoxime ammonia complex. The sulfide is oxidized to sulfur. Sulfite interferes and shall be precipitated with barium chloride. Thiosulfate is not titrated under the conditions of the determination.(b) Apparatus: burrette, 10 ml.(2) REAGENT. (a) 0.1 Preparation of 0.02 N potassium ferricyanide; -32.925 g. per liter - this solution must be kept in the dark. Weigh to the nearest tenth of a gram 6.6 g. of analytical reagent grade potassium ferricyanide and dissolve in one liter distilled water. Store in an amber bottle in the dark. Prepare fresh each week.(b) Standardization of ferricyanide solution: Transfer 50 ml. of solution to a 250 ml. Erlenmeyer flask. Add several crystals of potassium iodide, about one g., mix gently to dissolve, add one ml. of 6N hydrochloric acid, stopper the flask, and swirl gently. Let stand for 2 minutes, add 10 ml. of a 30% zinc sulfate solution, and titrate the mixture containing the gelatinous precipitate with standardized sodium thiosulfate or phenylarsine oxide titrant in the range of 0.025-0.050N. Add one ml. of starch indicator solution after the color has faded to a pale yellow, and continue the titration to the disappearance of the blue color. Calculate the normality of the ferricyanide solution using the equation: (c) Preparation of 6M ammonium chloride buffer, pH 9.3: Dissolve 200 g. ammonium chloride in approximately 500 ml. distilled water, add 200 ml. 14M reagent grade ammonium hydroxide and make up to one liter with distilled water. The buffer may be prepared in a hood. Store in a tightly stoppered container.(d) Preparation of 0.05M barium chloride solution: Dissolve 12-13 g. barium chloride dihydrate in one liter of distilled water.(e) Preparation of ferrous dimethylglyoxime indicator solution: Mix 10 ml. 0.6 percent ferrous sulfate, 50 ml. one percent dimethylglyoxime in ethanol, and 0.5 ml. concentrated sulfuric acid.(f) Preparation of stock sulfide standard, 1000 ppm: Dissol ve 2.4 g. reagent grade sodium sulfide in one liter of distilled water. Store in a tightly stoppered container. Diluted working standards must be prepared fresh daily and their concentrations determined by EPA test procedure 376.1 (see 40 CFR 136.3, Table IB, parameter 66 (49 FR 43234, October 26, 1984, with correction notice at 50 FR 690, January 4, 1985)) immediately prior to use.(g) Preparation of 10N NaOH: Dissolve 400 g. of analytical reagent grade NaOH in one liter distilled water.(h) Sample preservation and storage: samples are to be field filtered by either gravity or pressure with coarse filter paper, such as Whatman 4 or equivalent, immediately after collection. Filtered samples must be preserved by adjustment to pH> 12 with 10N NaOH. Sample containers must be covered tightly and stored at 4 degrees C until analysis. Samples shall be analyzed within 48 hours of collection. If these procedures cannot be achieved, it is the laboratory's responsibility to institute quality control procedures that shall provide documentation of sample integrity.(3) PROCEDURE. (a) Transfer 100 ml. of sample to be analyzed, or a suitable portion containing not more than 15 mg. sulfide supplemented to 100 ml. with distilled water, to a 250 ml. Erlenmeyer flask.(b) Adjust the sample to pH 8.5-9.5 with 6N HC1.(c) Add 20 ml. of 6M ammonium chloride buffer (pH 9.3), one ml. of ferrous dimethylglyoxime indicator, and 25 ml. of 0.05M barium chloride. Mix gently, stopper, and let stand for 10 minutes.(d) After 10 minutes titrate with standardized potassium ferricyanide to disappearance of pink color. The endpoint is reached when there is no reappearance of the pink color after 30 seconds.(e) Calculation and reporting of results. where
A=volume in ml. of potassium ferricyanide solution used, and
B= normality of potassium ferricyanide solution.
Report results to 2 significant figures.
(4) QUALITY CONTROL. (a) Each laboratory that uses this method is required to operate a formal quality control program. The minimum requirements of this program consist of an initial demonstration of laboratory capability and the analysis of replicate and spiked samples as a continuing check on performance. The laboratory is required to maintain performance records to define the quality of data that is generated. Ongoing performance checks shall be compared with established performance criteria to determine if the results of analyses are within precision and accuracy limits expected of the method.(b) Before performing any analyses, the analyst shall demonstrate the ability to generate acceptable precision and accuracy with this method by performing the following operations. 1. Perform 4 replicate analyses of a 20 mg/l sulfide standard prepared in distilled water. See sub. (2) (f). 2.a. Calculate clean water precision and accuracy in accordance with standard statistical procedures. Clean water acceptance limits are presented in subd. 2. b. These criteria shall be met or exceeded before sample analyses can be initiated. A clean water standard shall be analyzed with each sample set and the established criteria met for the analysis to be considered under control.b. Clean water precision and accuracy acceptance limits: for distilled water samples containing from 5 mg/l. to 50 mg/l. sulfide, the mean concentration from 4 replicate analyses shall be within the range of 50 to 110% of the true value.(c) The method detection limits or MDL may be determined periodically by each participating laboratory in accordance with the procedures specified in "Methods for Chemical Analysis of Municipal and Industrial Wastewater," EPA- 660/4-82-057, July 1982, EMSL, Cincinnati, OH 45268. For the convenience of the user, these procedures are contained in s. NR 252.0365.(d) A minimum of one spiked and one duplicate sample shall be performed for each analytical event, or 5% spikes and 5% duplicates when the number of samples per event exceeds 20. Spike levels are to be at the MDL and at x where x is the concentration found if in excess of the MDL. See par. (c) for MDL samples. Spike recovery shall be 40 to 120% for the analysis of a particular matrix type to be considered valid. If a sample or matrix type provides performance outside these acceptance limits, the analyses shall be repeated using the modified Monier-Williams procedures described in s. NR 252.036.(e) Report results in mg/liter. When duplicate and spiked samples are analyzed, report all data with the sample results.Wis. Admin. Code Department of Natural Resources § NR 252.035
Cr. Register, May, 2001, No. 545, eff. 6-1-01.