Methodology Category | Method1 | Citation1 |
Total Coliform Bacteria Methods: | ||
Lactose Fermentation Methods | Standard Total Coliform Fermentation Technique | Standard Methods 9221 B.1, B.2 (20th, 21st, and 22nd ed.)2,3 Standard Methods Online 9221 B.1, B.2-99, B-062.3 |
Presence-Absence (P-A) Coliform Test | Standard Methods 9221 D.l, D.2 (20th and 21st ed.)2,7 Standard Methods Online 9221 D.1, D.2-992,7 | |
Membrane Filtration Methods | Standard Total Coliform Membrane Filter Procedure | Standard Methods 9222 B, C (20th and 21st ed.)2,4 Standard Methods Online 9222 B-9729222 C-972, 4 |
Membrane Filtration using MI Medium | EPA Method 16042 | |
m-ColiBlue24 Test2, 4 | ||
Chromocult2, 4 | ||
Enzyme Substrate Methods | Colilert | Standard Methods 9223 B (20th, 21st and 22nd ed.)2,5 Standard Methods Online 9223 B-97, B-042, 5 |
Colilert-18 | Standard Methods 9223 B (21st and 22nd ed.)2,5 Standard Methods Online 9223 B-042,5 | |
Colisure | Standard Methods 9223 B (20th, 21st and 22nd ed.)2,5,6 Standard Methods Online 9223 B-97, B-042,5, 6 | |
E*Colite Test2 | ||
Readycult Test2 | ||
modified Colitag Test2 | ||
Tecta EC/TC Test2 | ||
Escherichia coli (E. coli) Methods: | ||
Escherichia coli Procedures (following Lactose Fermentation Methods) | EC-MUG Medium | Standard Methods 9221 F.1 (20th, 21st and 22nd ed.)2 Standard Methods Online 9221 F-062 |
Escherichia coli Partition Method | EC broth with MUG (EC-MUG) | Standard Methods 9222 G.lc(2) (20th and 21st ed.)2,8 |
NA-MUG Medium | Standard Methods 9222 G.lc(1) (20th and 21st ed.)2 | |
Membrane Filtration Methods | Membrane Filtration using MI Medium | EPA Method 16042 |
m-ColiBlue24 Test2. 4 | ||
Chromocult2. 4 | ||
Enzyme Substrate Methods | Colilert | Standard Methods 9223 B (20th, 21st and 22nd ed.)2,5 Standard Methods Online 9223 B-97, B-042.5, 6 |
Colilert-18 | Standard Methods 9223 B (21st and 22nd ed.)2.5 Standard Methods Online 9223 B-042, 5 | |
Colisure | Standard Methods 9223 B (20th, 21st and 22nd ed.)2,5,6 Standard Methods Online 9223 B-97, 042,5, 6. | |
E*Colite Test2 | ||
Readycult Test2 | ||
modified Colitag Test2 | ||
Tecta EC/TC Test2 |
1The procedures must be done in accordance with the documents listed in 41.2(1)"a"(6). For Standard Methods, either the 20th (1998) or 21st (2005) edition may be used. For Standard Methods Online, the year in which each method was approved by the Standard Methods Committee is designated by the last two digits following the hyphen in the method number. The methods listed are the only online versions that may be used. For vendor methods, the date of the method listed in 41.2(1) "a "(6) is the date/version of the approved method. The methods listed are the only versions that may be used for compliance with this rule. Laboratories should be careful to use only the approved versions of the methods, as product package inserts may not be the same as the approved versions of the methods.
2incorporated by reference. See 41.2(1)"a"(6).
3Lactose broth, as commercially available, may be used in lieu of lauryl tryptose broth if the system conducts at least 25 parallel tests between lactose broth and lauryl tryptose broth using the water normally tested and if the findings from this comparison demonstrate that the false-positive rate and the false-negative rate for total coliforms, using lactose broth, is less than 10 percent.
4A11 filtration series must begin with membrane filtration equipment that has been sterilized by autoclaving. Exposure of filtration equipment to UV light is not adequate to ensure sterilization. Subsequent to the initial autoclaving, exposure of the filtration equipment to UV light may be used to sanitize the funnels between nitrations within a filtration series. Alternatively, membrane filtration equipment that is presterilized by the manufacturer (i.e., disposable funnel units) may be used.
5Multiple-tube and multi-well enumerative formats for this method are approved for use in presence-absence determination under this subrule.
6Colisure results may be read after an incubation time of 24 hours.
7A multiple-tube enumerative format, as described in Standard Methods for the Examination of Water and Wastewater 9221, is approved for this method for use in presence-absence determination under this subrule.
8The following changes must be made to the EC broth with MUG (EC-MUG) formulation: Potassium dihydrogen phosphate, KH2PO4, must be 1.5 g, and 4-methylumbelliferyl-beta-D-glucuronide must be 0.05 g.
* Standard Methods 9221, "Multiple-Tube Fermentation Technique for Members of the Coliform Group," B.1, B.2, "Standard Total Coliform Fermentation Technique."
* Standard Methods 9221, "Multiple-Tube Fermentation Technique for Members of the Coliform Group," D.1, D.2, "Presence-Absence (P-A) Coliform Test."
* Standard Methods 9222, "Membrane Filter Technique for Members of the Coliform Group,"
B,"Standard Total Coliform Membrane Filter Procedure."
* Standard Methods 9222, "Membrane Filter Technique for Members of the Coliform Group,"
C,"Delayed-Incubation Total Coliform Procedure."
* Standard Methods 9223, "Enzyme Substrate Coliform Test," B, "Enzyme Substrate Test," Colilert and Colisure.
* Standard Methods 9221, "Multiple-Tube Fermentation Technique for Members of the Coliform Group," Fl,"Escherichia coli Procedure: EC-MUG Medium."
* Standard Methods 9222, "Membrane Filter Technique for Members of the Coliform Group," G.lc(2),"Escherichia coli Partition Method: EC Broth with MUG (EC-MUG)."
* Standard Methods 9222, "Membrane Filter Technique for Members of the Coliform Group," G.lc(1),"Escherichia coli Partition Method: NA-MUG Medium."
* Standard Methods 9221, "Multiple-Tube Fermentation Technique for Members of the Coliform Group," B.1, B.2, "Standard Total Coliform Fermentation Technique."
* Standard Methods 9221, "Multiple-Tube Fermentation Technique for Members of the Coliform Group," D.1, D.2, "Presence-Absence (P-A) Coliform Test."
* Standard Methods 9221, "Membrane Filter Technique for Members of the Coliform Group,"
B, "Standard Total Coliform Membrane Filter Procedure."
* Standard Methods 9222, "Membrane Filter Technique for Members of the Coliform Group,"
C,"Delayed-Incubation Total Coliform Procedure."
* Standard Methods 9223, "Enzyme Substrate Coliform Test," B, "Enzyme Substrate Test," Colilert and Colisure.
* Standard Methods 9221, "Multiple-Tube Fermentation Technique for Members of the Coliform Group," Fl,"Escherichia coli Procedure: EC-MUG Medium."
* Standard Methods 9222, "Membrane Filter Technique for Members of the Coliform Group," G.l.c(2),"Escherichia coli Partition Method: EC Broth with MUG (EC-MUG)."
* Standard Methods 9222, "Membrane Filter Technique for Members of the Coliform Group," G.l.c(1),"Escherichia coli Partition Method: NA-MUG Medium."
* Standard Methods Online 9221, "Multiple-Tube Fermentation Technique for Members of the Coliform Group" (1999), B.1, B.2-99, B-06, "Standard Total Coliform Fermentation Technique."
* Standard Methods Online 9221, "Multiple-Tube Fermentation Technique for Members of the Coliform Group" (1999), D.1, D.2-99, "Presence-Absence (P-A) Coliform Test."
* Standard Methods Online 9222, "Membrane Filter Technique for Members of the Coliform Group" (1997), B-97, "Standard Total Coliform Membrane Filter Procedure."
* Standard Methods Online 9222, "Membrane Filter Technique for Members of the Coliform Group" (1997), C-97, "Delayed-Incubation Total Coliform Procedure."
* Standard Methods Online 9223, "Enzyme Substrate Coliform Test" (1997), B-97, "Enzyme Substrate Test," Colilert and Colisure.
* Chromocult-"Chromocult Coliform Agar Presence/Absence Membrane Filter Test Method for Detection and Identification of Coliform Bacteria andEscherichia coli for Finished Waters," November 2000, Version 1.0.
* Readycult-"Readycult Coliforms 100 Presence/Absence Test for Detection and Identification of Coliform Bacteria andEscherichia coli in Finished Waters," January 2007, Version 1.1.
* Standard Methods 9221, "Multiple-Tube Fermentation Technique for Members of the Coliform Group," B.1, B.2, "Standard Total Coliform Fermentation Technique."
* Standard Methods 9223, "Enzyme Substrate Coliform Test," B, "Enzyme Substrate Test," Colilert and Colisure.
* Standard Methods 9221, "Multiple-Tube Fermentation Technique for Members of the Coliform Group," Fl,"Escherichia coli Procedure: EC-MUG Medium."
* Map of the distribution system served by the system;
* List of routine compliance sample locations for each sample period;
* List of repeat compliance sample locations for each routine compliance sample location;
* Any other sample locations necessary to meet the requirements of this subrule;
* Sample collection schedule;
* Proper sampling technique instructions;
* Log of samples taken; and
* For groundwater systems subject to 567-417. (455B), triggered source water monitoring plan.
* Any surface water or IGW PWS must meet the requirements for minimum residual disinfectant entering the distribution system pursuant to 567-paragraph 43.5(4)"b" (2)"1"; and
* A system that uses surface water or IGW must comply with the requirements specified in 567-paragraph 43.5(4)"j."3"(2)"2" for daily distribution system residual disinfectant monitoring. The system must measure and record the free and total chlorine residual disinfectant concentration at least at the same points in the distribution system and at the same time as routine and repeat total coliform bacteria samples are collected, as specified in 41.2(1)"e " through 41.2(1)"j." The residual disinfectant measurements required as a part of this subrule may be used to satisfy the requirement in 567-paragraph 43.5(4)"b" (2)"2" on the day(s) when a routine or repeat total coliform bacteria sample(s) is collected, in lieu of separate samples. The system shall report the residual disinfectant concentration to the laboratory with the bacteria sample and comply with the applicable reporting requirements of 567-subrule 42.4(3).
The decision and supporting rationale for invalidating a total coliform-positive sample under 41.2(1)"d" (1) must be documented in writing, and approved and signed by the supervisor of the water supply operations section or water supply engineering section and the department official who recommended the decision. The department must make this document available to EPA and the public. The written documentation must state the specific cause of the total coliform-positive sample and what action the system has taken, or will take, to correct this problem. The department may not invalidate a total coliform-positive sample solely on the grounds that all repeat samples are total coliform-negative or because of poor sampling technique.
* The system has anE. coli MCL violation.
* The system triggers one Level 2 assessment under the provisions of 41.2(1)"/" in a rolling 12-month period.
* The system triggers two Level 1 assessments under the provisions of 41.2(1)"l" in a rolling 12-month period.
* The system has a coliform treatment technique violation.
* The system has two coliform monitoring violations in a rolling 12-month period.
* The system has one monitoring coliform violation and one Level 1 assessment under the provisions of 41.2(1)"I" in a rolling 12-month period.
Population Served | Minimum Number of Routine Samples per Month |
1,001 to 2,500 | 2 |
2,501 to 3,300 | 3 |
3,301 to 4,100 | 4 |
4,101 to 4,900 | 5 |
4,901 to 5,800 | 6 |
5,801 to 6,700 | 7 |
6,701 to 7,600 | 8 |
7,601 to 8,500 | 9 |
8,501 to 12,900 | 10 |
12,901 to 17,200 | 15 |
17,201 to 21,500 | 20 |
21,501 to 25,000 | 25 |
25,001 to 33,000 | 30 |
33,001 to 41,000 | 40 |
41,001 to 50,000 | 50 |
50,001 to 59,000 | 60 |
59,001 to 70,000 | 70 |
70,001 to 83,000 | 80 |
83,001 to 96,000 | 90 |
96,001 to 130,000 | 100 |
130,001 to 220,000 | 120 |
220,001 to 320,000 | 150 |
320,001 to 450,000 | 180 |
450,001 to 600,000 | 210 |
600,001 to 780,000 | 240 |
780,001 to 970,000 | 270 |
970,001 to 1,230,000 | 300 |
Miles of Pipe | Minimum Number of Routine Samples per Month |
0-9 | 1 |
10-25 | 2 |
26-49 | 3 |
50-81 | 4 |
82 - 121 | 5 |
122 - 169 | 6 |
170-225 | 7 |
226 - 289 | 8 |
290-361 | 9 |
362 - 441 | 10 |
442 - 529 | 11 |
530 - 625 | 12 |
626 - 729 | 13 |
730 - 841 | 14 |
842 - 961 | 15 |
962 - 1,089 | 16 |
1,090-1,225 | 17 |
1,226-1,364 | 18 |
1,365-1,521 | 19 |
1,522-1,681 | 20 |
1,682-1,849 | 21 |
1,850-2,025 | 22 |
2,026 - 2,209 | 23 |
2,210-2,401 | 24 |
2,402-2,601 | 25 |
2,602 - 2,809 | 26 |
2,810-3,025 | 27 |
3,026 - 3,249 | 28 |
3,250-3,481 | 29 |
3,482-3,721 | 30 |
3,722 - 3,969 | 31 |
3,970-4,225 | 32 |
4,226 - 4,489 | 33 |
4,490-4,671 | 34 |
4,672-5,041 | 35 |
5,042 - 5,329 | 36 |
5,330 - 5,625 | 37 |
5,626 - 5,929 | 38 |
5,930-6,241 | 39 |
6,242-6,561 | 40 |
6,562 and greater | 41 |
Iowa Admin. Code r. 567-41.2