Fla. Admin. Code R. 61DER21-2

Current through Reg. 51, No. 009; January 14, 2025

Section 61DER21-2 - Testing Methodologies; Measurement Uncertainties; Screening Limits

In addition to the rules that exist under Rule 61D-6.008, Florida Administrative Code, the following procedures are promulgated on an emergency basis:

(1) Testing Methodologies: Methodologies used to screen for and confirm the administration of medications, drugs, and naturally occurring substances in horses shall be limited to the following: Immunoassay, Liquid Chromatography Mass Spectrometry (LCMS), Gas Chromatography Mass Spectrometry (GCMS), and/or Specific Gravity.

(2) Measurement Uncertainties: The established levels utilized by the division laboratory are as follows:

SUBSTANCE

MEASUREMENT UNCERTAINTY

(a) acepromazine [2-(1-hydroxyethyl) promazine sulfoxide] (b) albuterol (c) betamethasone (d) butorphanol (e) clenbuterol (f) dantrolene (5-hydroxydantrolene) (g) detomidine (carboxydetomidine) (h) dexamethasone (i) diclofenac (j) dimethyl sulfoxide (DMSO) (k) firocoxib (l) furosemide (m) glycopyrrolate (n) isoflupredone (o) lidocaine (OH-lidocaine) (p) mepivacaine (hydroxymepivacaine) (q) methocarbamol (r) methylprednisolone (s) omeprazole (t) prednisolone (u) procaine penicillin (v) triamcinolone acetonide (w) xylazine (x) ketoprofen (z) phenylbutazone (aa) flunixin (ab) boldenone (ac) testosterone (ad) nandrolone (5[ALPHA]-oestrane-3[BETA],17[ALPHA]-diol) (ae) stanozolol, 16[BETA]-hydroxystanozolol

No greater than +/- 1 ng/ml (urine) No greater than +/- 0.05 ng/ml (urine) No greater than +/- 6 pg/ml (serum) No greater than +/- 0.4 ng/ml (serum) No greater than +/- 30 pg/ml (urine) No greater than +/- 3.2 pg/ml (serum) No greater than +/- 12 pg/ml (serum) No greater than +/- 2.9 pg/ml (urine) No greater than +/- 5 pg/ml (serum) No greater than +/- 0.4 ng/ml (serum) No greater than +/- 1 [MICRO]g/ml (serum) No greater than +/- 2.1 ng/ml (serum) No greater than +/- 4.4 ng/ml (serum) No greater than +/- 0.6 pg/ml (serum) No greater than +/- 27 pg/ml (serum) No greater than +/- 1.8 pg/ml (serum) No greater than +/- 1.5 ng/ml (urine) No greater than +/- 0.05 ng/ml (serum) No greater than +/- 16 pg/ml (serum) No greater than +/- 0.09 ng/ml (urine) No greater than +/- 0.2 ng/ml (serum) No greater than +/- 10 ng/ml (serum) No greater than +/- 20 pg/ml (serum) No greater than +/- 1.6 pg/ml (serum) No greater than +/- 0. 21 ng/ml (primary; serum) No greater than +/- 0.15 ng/ml (secondary; serum) No greater than +/- 0.3 [MICRO]g/ml (primary; serum) No greater than +/- 0.025 [MICRO]g/ml (secondary; serum) No greater than +/- 1.9 ng/ml (primary; serum) No greater than +/- 0.13 ng/ml (secondary; serum) No greater than +/- 2.5 ng/ml (urine; male horses) No greater than +/- 11 ng/ml (urine) No greater than +/- 2.2 ng/ml (urine; male horses) No greater than +/- 0.17 ng/ml (urine)

If a sample is diluted for testing, the measurement uncertainty is multiplied by the factor of dilution.

(3) Screening Limit for Prohibited Substances in Horses: Any and all findings above zero that are over the limit of detection, under any condition of use, for prohibited substances shall be considered a violation of Section 550.2415, F.S.

Fla. Admin. Code Ann. R. 61DER21-2

Rulemaking Authority 550.0251(3), (11) 550.2415 (12) FS. Law Implemented 550.0251, 550.2415 FS.

Adopted by Florida Register Volume 47, Number 046, March 9, 2021 effective 3/4/2021.